Regulatory

Part:BBa_K1955003

Designed by: Justine Hsu   Group: iGEM16_CGU_Taiwan   (2016-10-14)

pSB1C3-5'HYG

We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.

(1) The basic part checked by PCR:

We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8％ agarose gel by electrophoresis.





Sequence and Features BBa_K1955003 SequenceAndFeatures

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